Effects of Propolis on Oxidative Stress in Rabbits Undergoing Implant Surgery

Ezgi Aydın; Ceylan Hepokur; Sema Mısır; Hasan Yeler

doi:10.7126/cumudj.356554

Research Article

Effects of Propolis on Oxidative Stress in Rabbits Undergoing Implant Surgery

Year 2018, Volume: 21 Issue: 2, 136 - 144, 20.07.2018

Ezgi Aydın , Ceylan Hepokur , Sema Mısır Hasan Yeler

https://doi.org/10.7126/cumudj.356554

Cited By: 4

Abstract

Abstract

Objectives: Implants are the natural or artificial
materials that replace a missing tissue or organ. When that missing part is one
or more teeth, there is a need for the reconstructive material that supports
prosthetic restoration, which is called dental implant. Many local and general
factors affect bone healing. Recent studies show that, free radicals are among
the factors that affect the bone healing. This study is planned presuming
propolis could have positive effects on oxidative stress and bone healing after
implantation due to its antioxidant nature, its effects on accelerating the
bone formation, wound healing and increasing the bone density.

Materials
and Methods: 24 New
Zealand white rabbits were used in the study. Animals were divided into 3
groups, each consisting of 8 rabbits. Implants were placed into the proximal
region of tibia unilaterally. Propolis was applied to slots before placing the
implants on local group and systemically applied after implantation on systemic
group.

Results: SOD activity was increased in both propolis groups
compared to control group, but it was not statistically significant. Catalase
level of the systemic group was found to be significantly higher. MDA level in
serum, which indicates lipid peroxidation, was significantly lower in both
propolis groups compared to control group. Reduced GSH concentration in
granulation tissue was increased in rabbit groups treated with propolis
compared to control group. There were no significant differences in calcium and
phosphorus values, however it can be said that there is a significant increase
in vitamin D amount of local and systemic groups.

Conclusion: Propolis has antioxidant, antimicrobial and many other
biological activities. Due to the features that it has, propolis has a positive
effect on osseointegration and bone healing. The comparison of systemic and
local group showed that, systemically given propolis provided better results
than local application.

Keywords

implant, antioxidant activity, propolis

References

1. Li, S., Yang, Y., Yu, C., Yao, Y., Wu, Y., Qian, L., & Cheung, C. W. (2015). Dexmedetomidine analgesia effects in patients undergoing dental implant surgery and its impact on postoperative inflammatory and oxidative stress. Oxidative medicine and cellular longevity, 2015.
2. Gvetadze, R., Krechina, E. K., Abramian, S. V., Ivanov, A. A., & Nubarian, A. P. (2013). Study of blood circulation microdynamics in gingival mucosa after dental implantation with the use of custom-made healing abutments. Stomatologiia, 92(3), 109.
3. Vlahovic, Z., Markovic, A., Golubovic, M., Scepanovic, M., Kalanovic, M., & Djinic, A. (2015). Histopathological comparative analysis of peri‐implant soft tissue response after dental implant placement with flap and flapless surgical technique. Experimental study in pigs. Clinical oral implants research, 26(11), 1309-1314.
4. Figueiredo, A., Coimbra, P., Cabrita, A., Guerra, F., & Figueiredo, M. (2013). Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response. Materials Science and Engineering: C, 33(6), 3506-3513.
5. Malet J., Mora F. & Bouchard P. (2012) Implant Dentistry at a Glance. Wiley-Blackwell; 144 p.
6. Kökçam, I., & Nazıroǧlu, M. (2002). Effects of vitamin E supplementation on blood antioxidants levels in patients with Behçet’s disease. Clinical biochemistry, 35(8), 633-639.
7. Gul, M., Kutay, F. Z., Temocin, S., & Hanninen, O. (2000). Cellular and clinical implications of glutathione.
8. Sato, N., Ueno, T., Kubo, K., Suzuki, T., Tsukimura, N., Att, W., ... & Ogawa, T. (2009). N-Acetyl cysteine (NAC) inhibits proliferation, collagen gene transcription, and redox stress in rat palatal mucosal cells. dental materials, 25(12), 1532-1540.
9. Thomas, M. V., & Puleo, D. A. (2011). Infection, inflammation, and bone regeneration: a paradoxical relationship. Journal of dental research, 90(9), 1052-1061.
10. Hold, G. L., & El-Omar, M. E. (2008). Genetic aspects of inflammation and cancer. Biochemical Journal, 410(2), 225-235.
11. Kalbacova, M., Roessler, S., Hempel, U., Tsaryk, R., Peters, K., Scharnweber, D., ... & Dieter, P. (2007). The effect of electrochemically simulated titanium cathodic corrosion products on ROS production and metabolic activity of osteoblasts and monocytes/macrophages. Biomaterials, 28(22), 3263-3272.
12. Lenz, R., Mittelmeier, W., Hansmann, D., Brem, R., Diehl, P., Fritsche, A., & Bader, R. (2009). Response of human osteoblasts exposed to wear particles generated at the interface of total hip stems and bone cement. Journal of Biomedical Materials Research Part A, 89(2), 370-378.
13. Tsaryk, R., Kalbacova, M., Hempel, U., Scharnweber, D., Unger, R. E., Dieter, P., ... & Peters, K. (2007). Response of human endothelial cells to oxidative stress on Ti6Al4V alloy. Biomaterials, 28(5), 806-813.
14. Tsukimura, N., Yamada, M., Aita, H., Hori, N., Yoshino, F., Lee, M. C. I., ... & Ogawa, T. (2009). N-acetyl cysteine (NAC)-mediated detoxification and functionalization of poly (methyl methacrylate) bone cement. Biomaterials, 30(20), 3378-3389.
15. Khansari, N., Shakiba, Y., & Mahmoudi, M. (2009). Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent patents on inflammation & allergy drug discovery, 3(1), 73-80.
16. Bereket, C., Özan, F., Sener, I., Tek, M., Altunkaynak, B. Z., Semirgin, S. U., ... & Özdemir, M. (2014). Propolis accelerates the consolidation phase in distraction osteogenesis. Journal of Craniofacial Surgery, 25(5), 1912-1916.
17. Bankova, V. (2005). Recent trends and important developments in propolis research. Evidence-based complementary and alternative medicine, 2(1), 29-32.
18. Banskota, A. H., Tezuka, Y., & Kadota, S. (2001). Recent progress in pharmacological research of propolis. Phytotherapy Research, 15(7), 561-571.
19. Bogdanov S (2012) Propolis composition healthy medicine a review bee product science; www.bee-hexagon.net.
20. Guney, A., Karaman, I., Oner, M., & Yerer, M. B. (2011). Effects of propolis on fracture healing: an experimental study. Phytotherapy Research, 25(11), 1648-1652.
21. Yu, L. W., Latriano, L., Duncan, S., Hartwick, R. A., & Witz, G. (1986). High-performance liquid chromatography analysis of the thiobarbituric acid adducts of malonaldehyde and trans, trans-muconaldehyde. Analytical biochemistry, 156(2), 326-333.
22. Sun, Y. I., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 34(3), 497-500.
23. Aebi HE (1983) Catalase. Methods of Enzymatic Analysis, 3, 273-286.
24. Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine, 70(1), 158-169.
25. Hobo S. Ichida E, Garcia LT (1991) Osseointegration and occlusal rehabilitation. 3rd ed. 33-62, Quintessence; Tokyo, 462 p.
26. De Smet, E., Jaecques, S., Vandamme, K., Vander Sloten, J., & Naert, I. (2005). Positive effect of early loading on implant stability in the bi‐cortical guinea‐pig model. Clinical oral implants research, 16(4), 402-407.
27. Sheweita, S. A., & Khoshhal, K. I. (2007). Calcium metabolism and oxidative stress in bone fractures: role of antioxidants. Current drug metabolism, 8(5), 519-525.
28. Ozmen, I., Naziroglu, M., & Okutan, R. (2006). Comparative study of antioxidant enzymes in tissues surrounding implant in rabbits. Cell biochemistry and function, 24(3), 275-281.
29. Södergren, E., Cederberg, J., Basu, S., & Vessby, B. (2000). Vitamin E supplementation decreases basal levels of F2-isoprostanes and prostaglandin F2α in rats. The Journal of nutrition, 130(1), 10-14.
30. Wohaieb, S. A., & Godin, D. V. (1987). Alterations in tissue antioxidant systems in the spontaneously diabetic (BB Wistar) rat. Canadian journal of physiology and pharmacology, 65(11), 2191-2195.
31. Aliciguzel, Y., Ozen, I., Aslan, M., & Karayalcin, U. (2003). Activities of xanthine oxidoreductase and antioxidant enzymes in different tissues of diabetic rats. Journal of Laboratory and Clinical Medicine, 142(3), 172-177.
32. Moreno, M. I. N., Isla, M. I., Sampietro, A. R., & Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71(1), 109-114.
33. Isla, M. I., Moreno, M. N., Sampietro, A. R., & Vattuone, M. A. (2001). Antioxidant activity of Argentine propolis extracts. Journal of Ethnopharmacology, 76(2), 165-170.
34. Koksel, O., Ozdulger, A., Tamer, L., Cinel, L., Ercil, M., Degirmenci, U., ... & Kanik, A. (2006). Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats. Pulmonary pharmacology & therapeutics, 19(2), 90-95.
35. Hoşnuter, M., Gürel, A., Babucçu, O., Armutcu, F., Kargi, E., & Işikdemir, A. (2004). The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns, 30(2), 121-125.
36. Di Carlo, G., Mascolo, N., Izzo, A. A., & Capasso, F. (1999). Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life sciences, 65(4), 337-353.

Year 2018, Volume: 21 Issue: 2, 136 - 144, 20.07.2018

Ezgi Aydın , Ceylan Hepokur , Sema Mısır Hasan Yeler

https://doi.org/10.7126/cumudj.356554

Cited By: 4

Abstract

References

1. Li, S., Yang, Y., Yu, C., Yao, Y., Wu, Y., Qian, L., & Cheung, C. W. (2015). Dexmedetomidine analgesia effects in patients undergoing dental implant surgery and its impact on postoperative inflammatory and oxidative stress. Oxidative medicine and cellular longevity, 2015.
2. Gvetadze, R., Krechina, E. K., Abramian, S. V., Ivanov, A. A., & Nubarian, A. P. (2013). Study of blood circulation microdynamics in gingival mucosa after dental implantation with the use of custom-made healing abutments. Stomatologiia, 92(3), 109.
3. Vlahovic, Z., Markovic, A., Golubovic, M., Scepanovic, M., Kalanovic, M., & Djinic, A. (2015). Histopathological comparative analysis of peri‐implant soft tissue response after dental implant placement with flap and flapless surgical technique. Experimental study in pigs. Clinical oral implants research, 26(11), 1309-1314.
4. Figueiredo, A., Coimbra, P., Cabrita, A., Guerra, F., & Figueiredo, M. (2013). Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response. Materials Science and Engineering: C, 33(6), 3506-3513.
5. Malet J., Mora F. & Bouchard P. (2012) Implant Dentistry at a Glance. Wiley-Blackwell; 144 p.
6. Kökçam, I., & Nazıroǧlu, M. (2002). Effects of vitamin E supplementation on blood antioxidants levels in patients with Behçet’s disease. Clinical biochemistry, 35(8), 633-639.
7. Gul, M., Kutay, F. Z., Temocin, S., & Hanninen, O. (2000). Cellular and clinical implications of glutathione.
8. Sato, N., Ueno, T., Kubo, K., Suzuki, T., Tsukimura, N., Att, W., ... & Ogawa, T. (2009). N-Acetyl cysteine (NAC) inhibits proliferation, collagen gene transcription, and redox stress in rat palatal mucosal cells. dental materials, 25(12), 1532-1540.
9. Thomas, M. V., & Puleo, D. A. (2011). Infection, inflammation, and bone regeneration: a paradoxical relationship. Journal of dental research, 90(9), 1052-1061.
10. Hold, G. L., & El-Omar, M. E. (2008). Genetic aspects of inflammation and cancer. Biochemical Journal, 410(2), 225-235.
11. Kalbacova, M., Roessler, S., Hempel, U., Tsaryk, R., Peters, K., Scharnweber, D., ... & Dieter, P. (2007). The effect of electrochemically simulated titanium cathodic corrosion products on ROS production and metabolic activity of osteoblasts and monocytes/macrophages. Biomaterials, 28(22), 3263-3272.
12. Lenz, R., Mittelmeier, W., Hansmann, D., Brem, R., Diehl, P., Fritsche, A., & Bader, R. (2009). Response of human osteoblasts exposed to wear particles generated at the interface of total hip stems and bone cement. Journal of Biomedical Materials Research Part A, 89(2), 370-378.
13. Tsaryk, R., Kalbacova, M., Hempel, U., Scharnweber, D., Unger, R. E., Dieter, P., ... & Peters, K. (2007). Response of human endothelial cells to oxidative stress on Ti6Al4V alloy. Biomaterials, 28(5), 806-813.
14. Tsukimura, N., Yamada, M., Aita, H., Hori, N., Yoshino, F., Lee, M. C. I., ... & Ogawa, T. (2009). N-acetyl cysteine (NAC)-mediated detoxification and functionalization of poly (methyl methacrylate) bone cement. Biomaterials, 30(20), 3378-3389.
15. Khansari, N., Shakiba, Y., & Mahmoudi, M. (2009). Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent patents on inflammation & allergy drug discovery, 3(1), 73-80.
16. Bereket, C., Özan, F., Sener, I., Tek, M., Altunkaynak, B. Z., Semirgin, S. U., ... & Özdemir, M. (2014). Propolis accelerates the consolidation phase in distraction osteogenesis. Journal of Craniofacial Surgery, 25(5), 1912-1916.
17. Bankova, V. (2005). Recent trends and important developments in propolis research. Evidence-based complementary and alternative medicine, 2(1), 29-32.
18. Banskota, A. H., Tezuka, Y., & Kadota, S. (2001). Recent progress in pharmacological research of propolis. Phytotherapy Research, 15(7), 561-571.
19. Bogdanov S (2012) Propolis composition healthy medicine a review bee product science; www.bee-hexagon.net.
20. Guney, A., Karaman, I., Oner, M., & Yerer, M. B. (2011). Effects of propolis on fracture healing: an experimental study. Phytotherapy Research, 25(11), 1648-1652.
21. Yu, L. W., Latriano, L., Duncan, S., Hartwick, R. A., & Witz, G. (1986). High-performance liquid chromatography analysis of the thiobarbituric acid adducts of malonaldehyde and trans, trans-muconaldehyde. Analytical biochemistry, 156(2), 326-333.
22. Sun, Y. I., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical chemistry, 34(3), 497-500.
23. Aebi HE (1983) Catalase. Methods of Enzymatic Analysis, 3, 273-286.
24. Paglia, D. E., & Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine, 70(1), 158-169.
25. Hobo S. Ichida E, Garcia LT (1991) Osseointegration and occlusal rehabilitation. 3rd ed. 33-62, Quintessence; Tokyo, 462 p.
26. De Smet, E., Jaecques, S., Vandamme, K., Vander Sloten, J., & Naert, I. (2005). Positive effect of early loading on implant stability in the bi‐cortical guinea‐pig model. Clinical oral implants research, 16(4), 402-407.
27. Sheweita, S. A., & Khoshhal, K. I. (2007). Calcium metabolism and oxidative stress in bone fractures: role of antioxidants. Current drug metabolism, 8(5), 519-525.
28. Ozmen, I., Naziroglu, M., & Okutan, R. (2006). Comparative study of antioxidant enzymes in tissues surrounding implant in rabbits. Cell biochemistry and function, 24(3), 275-281.
29. Södergren, E., Cederberg, J., Basu, S., & Vessby, B. (2000). Vitamin E supplementation decreases basal levels of F2-isoprostanes and prostaglandin F2α in rats. The Journal of nutrition, 130(1), 10-14.
30. Wohaieb, S. A., & Godin, D. V. (1987). Alterations in tissue antioxidant systems in the spontaneously diabetic (BB Wistar) rat. Canadian journal of physiology and pharmacology, 65(11), 2191-2195.
31. Aliciguzel, Y., Ozen, I., Aslan, M., & Karayalcin, U. (2003). Activities of xanthine oxidoreductase and antioxidant enzymes in different tissues of diabetic rats. Journal of Laboratory and Clinical Medicine, 142(3), 172-177.
32. Moreno, M. I. N., Isla, M. I., Sampietro, A. R., & Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71(1), 109-114.
33. Isla, M. I., Moreno, M. N., Sampietro, A. R., & Vattuone, M. A. (2001). Antioxidant activity of Argentine propolis extracts. Journal of Ethnopharmacology, 76(2), 165-170.
34. Koksel, O., Ozdulger, A., Tamer, L., Cinel, L., Ercil, M., Degirmenci, U., ... & Kanik, A. (2006). Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats. Pulmonary pharmacology & therapeutics, 19(2), 90-95.
35. Hoşnuter, M., Gürel, A., Babucçu, O., Armutcu, F., Kargi, E., & Işikdemir, A. (2004). The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns, 30(2), 121-125.
36. Di Carlo, G., Mascolo, N., Izzo, A. A., & Capasso, F. (1999). Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life sciences, 65(4), 337-353.

There are 36 citations in total.

Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	Original Research Articles
Authors	Ezgi Aydın Ceylan Hepokur Sema Mısır Hasan Yeler
Publication Date	July 20, 2018
Submission Date	November 20, 2017
Published in Issue	Year 2018Volume: 21 Issue: 2

Cite

EndNote	Aydın E, Hepokur C, Mısır S, Yeler H (July 1, 2018) Effects of Propolis on Oxidative Stress in Rabbits Undergoing Implant Surgery. Cumhuriyet Dental Journal 21 2 136–144.

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