Effect of Chlorhexidine Treatment on Microleakage of Glass Ionomer, Giomer and Methacrylate-Based Composite Restorations in Class V Cavities

Ayşe Aslı Şenol; Seda Özmen; Büşra Karabulut Gençer; Bilge Tarçın; Pınar Yılmaz Atalı

doi:10.52037/eads.2024.0004

Research Article

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Year 2024, Volume: 51 Issue: 1, 20 - 27, 30.04.2024

Ayşe Aslı Şenol Seda Özmen Büşra Karabulut Gençer Bilge Tarçın Pınar Yılmaz Atalı

https://doi.org/10.52037/eads.2024.0004

Abstract

References

1. N KS, K KS, Subba Reddy VV. Effect of Dentin Disinfection with 2 Int J Clin Pediatr Dent. 2017;10(3):223–228. doi:10.5005/jpjournals-10005-1440.
2. H F, F S, Sc A, B A. Combination Effect of Hemostatic and Disinfecting Agents on Micro-leakage of Restorations Bonded with Different Bonding Systems. J Dent Biomater. 2016;3(3):292– 298.
3. Amaral CM, Peris AR, Ambrosano GM, Pimenta LA. Microleakage and gap formation of resin composite restorations polymerized with different techniques. Am J Dent. 2004;17(3):156–60.
4. Tulunoglu O, Ayhan H, Olmez A, Bodur H. The effect of cavity disinfectants on microleakage in dentin bonding systems. J Clin Pediatr Dent. 1998;22(4):299–305.
5. Türkün M, Türkün LS, Kalender A. Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins. Quintessence Int. 2004;35(6):469–76.
6. Arslan S, Ertaş H, Zorba YO. Effect of a plant-based hemostatic agent on microleakage of self-etching adhesives. Med Oral Patol Oral Cir Bucal. 2013;18(1):e124–9. doi:10.4317/medoral.17959.
7. Harnirattisai C, Kuphasuk W, Senawongse P, Tagami J. Bond strengths of resin cements to astringent-contaminated dentin. Oper Dent. 2009;34(4):415–22. doi:10.2341/08-107.
8. Sharafeddin F, Farhadpour H. Evaluation of Shear Bond Strength of Total- and Self-etching Adhesive Systems after Application of Chlorhexidine to Dentin Contaminated with a Hemostatic Agent. J Dent (Shiraz). 2015;16(3):175–81.
9. Nedeljkovic I, Teughels W, De Munck J, Van Meerbeek B, Van Landuyt KL. Is secondary caries with composites a material-based problem? Dent Mater. 2015;31(11):e247–77. doi:10.1016/j.dental.2015.09.001.
10. Breschi L, Maravic T, Comba A, Cunha SR, Loguercio AD, Reis A, et al. Chlorhexidine preserves the hybrid layer in vitro after 10-years aging. Dent Mater. 2020;36(5):672–680. doi:10.1016/j.dental.2020.03.009.
11. Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc. 1986;112(6):863–9. doi:10.14219/jada.archive.1986.0118.
12. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res. 2005;84(8):741–6. doi:10.1177/154405910508400811.
13. Komori PC, Pashley DH, Tjäderhane L, Breschi L, Mazzoni A, de Goes MF, et al. Effect of 2 Oper Dent. 2009;34(2):157–65. doi:10.2341/08-55.
14. Lugassy D, Segal P, Blumer S, Eger M, Shely A, Matalon S. Effect of Two Traditional Polyacrylic Acid Conditioners and J Clin Pediatr Dent. 2018;42(4):287–291. doi:10.17796/1053-462842.4.8.
15. Itota T, Carrick TE, Yoshiyama M, McCabe JF. Fluoride release and recharge in giomer, compomer and resin composite. Dent Mater. 2004;20(9):789–95. doi:10.1016/j.dental.2003.11.009.
16. Rusnac ME, Gasparik C, Irimie AI, Grecu AG, Mesaroş A, Dudea D. Giomers in dentistry - at the boundary between dental composites and glass-ionomers. Med Pharm Rep. 2019;92(2):123– 128. doi:10.15386/mpr-1169.
17. Dionysopoulos D, Tolidis K, Gerasimou P, Koliniotou-Koumpia E. Effect of preheating on the film thick- ness of contemporary composite restorative materials. Journal of Dental Sciences. 2014;9(4):313–319. doi:https://doi.org/10.1016/j.jds.2014.03.006.
18. Bourbia M, Ma D, Cvitkovitch DG, Santerre JP, Finer Y. Cariogenic bacteria degrade dental resin composites and adhesives. J Dent Res. 2013;92(11):989–94. doi:10.1177/0022034513504436.
19. Tanner AC, Kressirer CA, Faller LL. Understanding Caries From the Oral Microbiome Perspective. J Calif Dent Assoc. 2016;44(7):437–46.
20. Banerjee A, Frencken JE, Schwendicke F, Innes NPT. Con- temporary operative caries management: consensus recom- mendations on minimally invasive caries removal. Br Dent J. 2017;223(3):215–222. doi:10.1038/sj.bdj.2017.672.
21. Bitello-Firmino L, Soares VK, Damé-Teixeira N, Parolo CCF, Maltz M. Microbial Load After Selective and Complete Caries Re- moval in Permanent Molars: a Randomized Clinical Trial. Braz Dent J. 2018;29(3):290–295. doi:10.1590/0103-6440201801816.
22. Bin-Shuwaish M, AlHussaini A, AlHudaithy L, AlDukhiel S, AlJamhan A, Alrahlah A. Effects of different antibacterial dis- infectants on microleakage of bulk-fill composite bonded to different tooth structures. BMC Oral Health. 2021;21(1):348. doi:10.1186/s12903-021-01717-7.
23. Boutsiouki C, Frankenberger R, Lücker S, Krämer N. Inhibition of secondary caries in vitro by addition of chlorhexidine to adhesive components.-CHX Effect on Microleakage in Class V Restorations Dent Mater. 2019;35(3):422–433. doi:10.1016/j.dental.2018.12.002.
24. Gou Y, Jin W, He Y, Luo Y, Si R, He Y, et al. Effect of Cavity Cleanser With Long-Term Antibacterial and Anti-Proteolytic Activities on Resin-Dentin Bond Stability. Front Cell Infect Microbiol. 2021;11:784153. doi:10.3389/fcimb.2021.784153.
25. Giacomini MC, Scaffa P, Chaves LP, Vidal C, Machado TN, Honório HM, et al. Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System. Oper Dent. 2017;42(6):E188–e196. doi:10.2341/16-178-l.
26. Mazzoni A, Tjäderhane L, Checchi V, Di Lenarda R, Salo T, Tay FR, et al. Role of dentin MMPs in caries progression and bond stability. J Dent Res. 2015;94(2):241–51. doi:10.1177/0022034514562833.
27. Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S, et al. Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater. 2013;29(1):116–35. doi:10.1016/j.dental.2012.08.004.
28. Takahashi N, Nyvad B. Ecological Hypothesis of Dentin and Root Caries. Caries Res. 2016;50(4):422–31. doi:10.1159/000447309.
29. Pradhan PK, Sahoo KC, Ghosh K, Lata S, Patri G, Bhol S. Does Cavity Disinfectant Affect Sealing Ability of Universal Self-etch Adhesive? J Contemp Dent Pract. 2021;22(3):273–277.
30. Loguercio AD, Hass V, Gutierrez MF, Luque-Martinez IV, Szezs A, Stanislawczuk R, et al. Five-year Effects of Chlorhexidine on the In Vitro Durability of Resin/Dentin Interfaces. J Adhes Dent. 2016;18(1):35–42. doi:10.3290/j.jad.a35514.
31. Fernandes GL, Strazzi-Sahyon HB, Suzuki TYU, Briso ALF, Dos Santos PH. Influence of Chlorhexidine Gluconate on the Immediate Bond Strength of a Universal Adhesive System on Dentine Subjected to Different Bonding Protocols: An In Vitro Pilot Study. Oral Health Prev Dent. 2020;18(1):71–76. doi:10.3290/j.ohpd.a43934.
32. Kimyai S, Mohammadi N, Bahari M, Pesyanian E, Pesyanian F. Effect of Cavity Disinfection with Chlorhexidine on Marginal Gap of Class V Composite Restorations Bonded with a Universal Adhesive Using Self-Etch and Etch-and-Rinse Bonding Strategy. Front Dent. 2020;17(3):1–7. doi:10.18502/fid.v17i1.3963.
33. Boaru MO, Tărăboan t, ă I, Stoleriu S, Andrian S, Pancu G, Nica I, et al. The Influence of Chlorhexidine Gluconate Dentine Pre-Treatment on Adhesive Interface and Marginal Sealing. Medicina (Kaunas). 2023;59(2). doi:10.3390/medicina59020278.
34. Gupta J, Thomas MS, Radhakrishna M, Srikant N, Ginjupalli K. Effect of silver diamine fluoride-potassium iodide and 2 J Conserv Dent. 2019;22(2):201–206. doi:10.4103/jcd.Jcd48518.
35. Mutluay AT, Mutluay M. Effects of Different Disinfection Methods on Microleakage of Giomer Restorations. Eur J Dent. 2019;13(4):569–573. doi:10.1055/s-0039-1698370.
36. Santini A, Ivanovic V, Ibbetson R, Milia E. Influence of cavity configuration on microleakage around Class V restorations bonded with seven self-etching adhesives. J Esthet Restor Dent. 2004;16(2):128–35; discussion 136. doi:10.1111/j.17088240.2004.tb00020.x.
37. Kapdan A, Öztaş N. Effects of chlorhexidine and gaseous ozone on microleakage and on the bond strength of dentin bonding agents with compomer restoration on primary teeth. Journal of Dental Sciences. 2015;10(1):46–54.
38. Thomas HA, Singh N, Thomas AM, Masih S, Cherian JM, Varghese KG. Effect of protective coating agents on microleakage and flexural strength of glass ionomer cement and zirconomer. an in vitro study. Eur Arch Paediatr Dent. 2024;25(1):57–63. doi:10.1007/s40368-023-00853-8.
39. Askar H, Krois J, Göstemeyer G, Bottenberg P, Zero D, Banerjee A, et al. Secondary caries: what is it, and how it can be controlled, detected, and managed? Clin Oral Investig. 2020;24(5):1869–1876. doi:10.1007/s00784-020-03268-7.
40. Yao C, Zhou L, Yang H, Wang Y, Sun H, Guo J, et al. Effectof silane pretreatment on the immediate bonding of universal adhesives to computer-aided design/computer-aided manufacturing lithium disilicate glass ceramics. Eur J Oral Sci.

Effect of Chlorhexidine Treatment on Microleakage of Glass Ionomer, Giomer and Methacrylate-Based Composite Restorations in Class V Cavities

Year 2024, Volume: 51 Issue: 1, 20 - 27, 30.04.2024

Ayşe Aslı Şenol Seda Özmen Büşra Karabulut Gençer Bilge Tarçın Pınar Yılmaz Atalı

https://doi.org/10.52037/eads.2024.0004

Abstract

Purpose: The aim of this in vitro study was to evaluate the effect of material selection and chlorhexidine digluconate (CHX) pretreatment on microleakage in Class V cavities restored with 3 different restorative materials.
Materials and Methods: Thirty freshly extracted sound human molars were cleaned and stored in distilled water. Sixty standard Class V cavities were prepared on gingival 1/3 of buccal and lingual surfaces of each tooth. Teeth were randomly assigned into 3 groups corresponding to the selected restorative material (Fuji II LC Capsule/FC, GC; Beautifil Flow Plus/BF, Shofu; and GrandioSo Flow/GF, VOCO). Lingual cavities were pre-treated with CHX (Cavity Cleanser, Bisco). The cavities were restored according to manufacturer's instructions using the materials' own adhesive systems. Teeth were stored in distilled water (24°C) for 6 months for aging. Specimens were immersed in 2% methylene blue solution for 1 h and sectioned longitudinally in buccolingual direction. Occlusal/gingival margins were examined for dye penetration and scored under x8 and x20 magnification using stereomicroscope (Leica MZ7.5, Leica Microsystems). Statistical analysis was performed using chi-square test with a significance level of p<0.05.
Results: CHX pretreatment did not result in a significant difference between microleakage values in gingival and occlusal margins of cavities restored with different materials (p>0.05). There was no statistically significant difference between the amount of microleakage of GF, FC and BF restorations in CHX-treated and untreated cavities (p>0.05).
Conclusion: Application of CHX as cavity cleaner could not prevent dye penetration in Class V cavities restored with giomer, glass ionomer, and resin composites.
Key Words: Chlorhexidine gluconate, composite resin, glass ionomer, microleakage.

Keywords

Chlorhexidine gluconate, composite resin, glass ionomer, microleakage.

Ethical Statement

Etik belgesi ek dosya olarak sisteme eklenmiştir.

References

1. N KS, K KS, Subba Reddy VV. Effect of Dentin Disinfection with 2 Int J Clin Pediatr Dent. 2017;10(3):223–228. doi:10.5005/jpjournals-10005-1440.
2. H F, F S, Sc A, B A. Combination Effect of Hemostatic and Disinfecting Agents on Micro-leakage of Restorations Bonded with Different Bonding Systems. J Dent Biomater. 2016;3(3):292– 298.
3. Amaral CM, Peris AR, Ambrosano GM, Pimenta LA. Microleakage and gap formation of resin composite restorations polymerized with different techniques. Am J Dent. 2004;17(3):156–60.
4. Tulunoglu O, Ayhan H, Olmez A, Bodur H. The effect of cavity disinfectants on microleakage in dentin bonding systems. J Clin Pediatr Dent. 1998;22(4):299–305.
5. Türkün M, Türkün LS, Kalender A. Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins. Quintessence Int. 2004;35(6):469–76.
6. Arslan S, Ertaş H, Zorba YO. Effect of a plant-based hemostatic agent on microleakage of self-etching adhesives. Med Oral Patol Oral Cir Bucal. 2013;18(1):e124–9. doi:10.4317/medoral.17959.
7. Harnirattisai C, Kuphasuk W, Senawongse P, Tagami J. Bond strengths of resin cements to astringent-contaminated dentin. Oper Dent. 2009;34(4):415–22. doi:10.2341/08-107.
8. Sharafeddin F, Farhadpour H. Evaluation of Shear Bond Strength of Total- and Self-etching Adhesive Systems after Application of Chlorhexidine to Dentin Contaminated with a Hemostatic Agent. J Dent (Shiraz). 2015;16(3):175–81.
9. Nedeljkovic I, Teughels W, De Munck J, Van Meerbeek B, Van Landuyt KL. Is secondary caries with composites a material-based problem? Dent Mater. 2015;31(11):e247–77. doi:10.1016/j.dental.2015.09.001.
10. Breschi L, Maravic T, Comba A, Cunha SR, Loguercio AD, Reis A, et al. Chlorhexidine preserves the hybrid layer in vitro after 10-years aging. Dent Mater. 2020;36(5):672–680. doi:10.1016/j.dental.2020.03.009.
11. Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc. 1986;112(6):863–9. doi:10.14219/jada.archive.1986.0118.
12. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res. 2005;84(8):741–6. doi:10.1177/154405910508400811.
13. Komori PC, Pashley DH, Tjäderhane L, Breschi L, Mazzoni A, de Goes MF, et al. Effect of 2 Oper Dent. 2009;34(2):157–65. doi:10.2341/08-55.
14. Lugassy D, Segal P, Blumer S, Eger M, Shely A, Matalon S. Effect of Two Traditional Polyacrylic Acid Conditioners and J Clin Pediatr Dent. 2018;42(4):287–291. doi:10.17796/1053-462842.4.8.
15. Itota T, Carrick TE, Yoshiyama M, McCabe JF. Fluoride release and recharge in giomer, compomer and resin composite. Dent Mater. 2004;20(9):789–95. doi:10.1016/j.dental.2003.11.009.
16. Rusnac ME, Gasparik C, Irimie AI, Grecu AG, Mesaroş A, Dudea D. Giomers in dentistry - at the boundary between dental composites and glass-ionomers. Med Pharm Rep. 2019;92(2):123– 128. doi:10.15386/mpr-1169.
17. Dionysopoulos D, Tolidis K, Gerasimou P, Koliniotou-Koumpia E. Effect of preheating on the film thick- ness of contemporary composite restorative materials. Journal of Dental Sciences. 2014;9(4):313–319. doi:https://doi.org/10.1016/j.jds.2014.03.006.
18. Bourbia M, Ma D, Cvitkovitch DG, Santerre JP, Finer Y. Cariogenic bacteria degrade dental resin composites and adhesives. J Dent Res. 2013;92(11):989–94. doi:10.1177/0022034513504436.
19. Tanner AC, Kressirer CA, Faller LL. Understanding Caries From the Oral Microbiome Perspective. J Calif Dent Assoc. 2016;44(7):437–46.
20. Banerjee A, Frencken JE, Schwendicke F, Innes NPT. Con- temporary operative caries management: consensus recom- mendations on minimally invasive caries removal. Br Dent J. 2017;223(3):215–222. doi:10.1038/sj.bdj.2017.672.
21. Bitello-Firmino L, Soares VK, Damé-Teixeira N, Parolo CCF, Maltz M. Microbial Load After Selective and Complete Caries Re- moval in Permanent Molars: a Randomized Clinical Trial. Braz Dent J. 2018;29(3):290–295. doi:10.1590/0103-6440201801816.
22. Bin-Shuwaish M, AlHussaini A, AlHudaithy L, AlDukhiel S, AlJamhan A, Alrahlah A. Effects of different antibacterial dis- infectants on microleakage of bulk-fill composite bonded to different tooth structures. BMC Oral Health. 2021;21(1):348. doi:10.1186/s12903-021-01717-7.
23. Boutsiouki C, Frankenberger R, Lücker S, Krämer N. Inhibition of secondary caries in vitro by addition of chlorhexidine to adhesive components.-CHX Effect on Microleakage in Class V Restorations Dent Mater. 2019;35(3):422–433. doi:10.1016/j.dental.2018.12.002.
24. Gou Y, Jin W, He Y, Luo Y, Si R, He Y, et al. Effect of Cavity Cleanser With Long-Term Antibacterial and Anti-Proteolytic Activities on Resin-Dentin Bond Stability. Front Cell Infect Microbiol. 2021;11:784153. doi:10.3389/fcimb.2021.784153.
25. Giacomini MC, Scaffa P, Chaves LP, Vidal C, Machado TN, Honório HM, et al. Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System. Oper Dent. 2017;42(6):E188–e196. doi:10.2341/16-178-l.
26. Mazzoni A, Tjäderhane L, Checchi V, Di Lenarda R, Salo T, Tay FR, et al. Role of dentin MMPs in caries progression and bond stability. J Dent Res. 2015;94(2):241–51. doi:10.1177/0022034514562833.
27. Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S, et al. Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins. Dent Mater. 2013;29(1):116–35. doi:10.1016/j.dental.2012.08.004.
28. Takahashi N, Nyvad B. Ecological Hypothesis of Dentin and Root Caries. Caries Res. 2016;50(4):422–31. doi:10.1159/000447309.
29. Pradhan PK, Sahoo KC, Ghosh K, Lata S, Patri G, Bhol S. Does Cavity Disinfectant Affect Sealing Ability of Universal Self-etch Adhesive? J Contemp Dent Pract. 2021;22(3):273–277.
30. Loguercio AD, Hass V, Gutierrez MF, Luque-Martinez IV, Szezs A, Stanislawczuk R, et al. Five-year Effects of Chlorhexidine on the In Vitro Durability of Resin/Dentin Interfaces. J Adhes Dent. 2016;18(1):35–42. doi:10.3290/j.jad.a35514.
31. Fernandes GL, Strazzi-Sahyon HB, Suzuki TYU, Briso ALF, Dos Santos PH. Influence of Chlorhexidine Gluconate on the Immediate Bond Strength of a Universal Adhesive System on Dentine Subjected to Different Bonding Protocols: An In Vitro Pilot Study. Oral Health Prev Dent. 2020;18(1):71–76. doi:10.3290/j.ohpd.a43934.
32. Kimyai S, Mohammadi N, Bahari M, Pesyanian E, Pesyanian F. Effect of Cavity Disinfection with Chlorhexidine on Marginal Gap of Class V Composite Restorations Bonded with a Universal Adhesive Using Self-Etch and Etch-and-Rinse Bonding Strategy. Front Dent. 2020;17(3):1–7. doi:10.18502/fid.v17i1.3963.
33. Boaru MO, Tărăboan t, ă I, Stoleriu S, Andrian S, Pancu G, Nica I, et al. The Influence of Chlorhexidine Gluconate Dentine Pre-Treatment on Adhesive Interface and Marginal Sealing. Medicina (Kaunas). 2023;59(2). doi:10.3390/medicina59020278.
34. Gupta J, Thomas MS, Radhakrishna M, Srikant N, Ginjupalli K. Effect of silver diamine fluoride-potassium iodide and 2 J Conserv Dent. 2019;22(2):201–206. doi:10.4103/jcd.Jcd48518.
35. Mutluay AT, Mutluay M. Effects of Different Disinfection Methods on Microleakage of Giomer Restorations. Eur J Dent. 2019;13(4):569–573. doi:10.1055/s-0039-1698370.
36. Santini A, Ivanovic V, Ibbetson R, Milia E. Influence of cavity configuration on microleakage around Class V restorations bonded with seven self-etching adhesives. J Esthet Restor Dent. 2004;16(2):128–35; discussion 136. doi:10.1111/j.17088240.2004.tb00020.x.
37. Kapdan A, Öztaş N. Effects of chlorhexidine and gaseous ozone on microleakage and on the bond strength of dentin bonding agents with compomer restoration on primary teeth. Journal of Dental Sciences. 2015;10(1):46–54.
38. Thomas HA, Singh N, Thomas AM, Masih S, Cherian JM, Varghese KG. Effect of protective coating agents on microleakage and flexural strength of glass ionomer cement and zirconomer. an in vitro study. Eur Arch Paediatr Dent. 2024;25(1):57–63. doi:10.1007/s40368-023-00853-8.
39. Askar H, Krois J, Göstemeyer G, Bottenberg P, Zero D, Banerjee A, et al. Secondary caries: what is it, and how it can be controlled, detected, and managed? Clin Oral Investig. 2020;24(5):1869–1876. doi:10.1007/s00784-020-03268-7.
40. Yao C, Zhou L, Yang H, Wang Y, Sun H, Guo J, et al. Effectof silane pretreatment on the immediate bonding of universal adhesives to computer-aided design/computer-aided manufacturing lithium disilicate glass ceramics. Eur J Oral Sci.

There are 40 citations in total.

Details

Primary Language	English
Subjects	Dental Materials and Equipment, Restorative Dentistry
Journal Section	Original Research Articles
Authors	Ayşe Aslı Şenol 0000-0003-3542-4877 Seda Özmen 0000-0002-5958-8828 Büşra Karabulut Gençer 0000-0002-2856-0662 Bilge Tarçın 0000-0002-9220-8671 Pınar Yılmaz Atalı 0000-0003-3121-360X
Early Pub Date	April 30, 2024
Publication Date	April 30, 2024
Submission Date	November 11, 2023
Acceptance Date	April 20, 2024
Published in Issue	Year 2024 Volume: 51 Issue: 1

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Vancouver	Şenol AA, Özmen S, Karabulut Gençer B, Tarçın B, Yılmaz Atalı P. Effect of Chlorhexidine Treatment on Microleakage of Glass Ionomer, Giomer and Methacrylate-Based Composite Restorations in Class V Cavities. EADS. 2024;51(1):20-7.

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