Research Article
BibTex RIS Cite

Efficacy of pure olive oil and PRF in the prevention of postoperative peritoneal adhesions

Year 2022, Volume: 47 Issue: 2, 570 - 579, 30.06.2022
https://doi.org/10.17826/cumj.1031388

Abstract

Purpose: In this study, we applied platelet rich fibrin (PRF) and pure olive oil on the incision surfaces of rats. We aimed to examine whether PRF may be used safely to prevent peritoneal adhesions.
Materials and Methods: Fourty rats were divided into 4 groups (n=8). Eight rats, not included in the study groups, were used to obtain PRF material. Group 1 had no surgical procedure, Group 2 was operated without medication, Group 3 was operated and received 1cc olive oil, Group 4 was operated and received 1 cc PRF. After 21 days, cecum areas were examined histopathologically. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and platelet-derived growth factor (PDGF) levels were measured in serum by ELISA.
Results: The adhesion scores and severity of fibrosis in Group 3 and 4 were significantly lower than Group 2. Plasma TNF-α value was significantly higher in Group 2 than Group 4. Plasma PDGF value was significantly higher in Group 2 than Group 3 and 4.
Discussion: PRF reduced intestinal adhesion by inhibiting the proliferation of fibroblasts and inflammatory cells, and promoting the proliferation of mesothelial cells. PRF has anti-inflammatory effect and prevented postop adhesions, based mainly on growth factors and cytokines in its content.

Thanks

Dear Editor-in-Chief, Please find enclosed our manuscript, “Investigation of the Efficacy of Pure Olive Oil and Prf in Preventıng Postoperative Peritoneal Adhesions in Rats" by Dilan Altıntaş Ural et al. which we would like to submit for publication as an original article in Cukurova Medical Journal. We believe this study is interesting to a global audience since it will make contributions to literature and serve well especially to paediatric surgeons. Thus with this features, we hope this article will be found suitable to be published in this precious journal. All of the authors declare that they have all participated in the design, execution, and analysis of the paper, and that they have approved the final version. Additionally, there are no conflicts of interest in connection with this paper and the material described is not under publication or consideration for publication elsewhere. Thank you for your valuable time.Yours sincerely, Corresponding Author: Dr.Dilan Altıntaş URAL, Assistant Professor, Md Mailing Address: Balgat Mahallesi, Mevlana Caddesi, 1422. Sokak, No: 4, 06520, Çankaya/Ankara E-mail: dilanaltintas@yahoo.com Contact Phone Number :+90 505 8120302

References

  • References : 1.Ten Broek RP, Strik C, Issa Y, Bleichrodt RP, van Goor H. Adhesiolysis-related morbidity in abdominal surgery. Ann Surg. 2013 Jul;258(1):98-106. doi: 10.1097/SLA.0b013e31826f4969. PMID: 23013804.
  • 2.Cai T, Qiu J, Ji Y, Li W, Ding Z, Suo C, Chang J, Wang J, He R, Qian Y, Guo X, Zhou L, Sheng H, Shen L, Qiu J. IL-17-producing ST2+ group 2 innate lymphoid cells play a pathogenic role in lung inflammation. J Allergy Clin Immunol. 2019 Jan;143(1):229-244.e9. doi: 10.1016/j.jaci.2018.03.007. Epub 2018 Apr 3. PMID: 29625134; PMCID: PMC6170730.
  • 3.Kırdak T, Uysal E, Korun N. Karın içi yapışıklıkların önlenmesinde metilprednizolonun farklı dozlarının etkinliğinin incelenmesi. Ulus Travma Acil Cerrahi Derg. 2008;14(3):188-91.
  • 4. Khan J, Chandler WL. Discordant Partial Thromboplastin Time (PTT) vs Anti-Xa Heparin Activity. Am J Clin Pathol. 2019 Mar 1;151(4):424-432. doi: 10.1093/ajcp/aqy156. PMID: 30475952.
  • 5.Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. 25. Slowrelease of growthfactorsand thrombospondin-1 in Chouk¬roun’splatelet-rich fibrin (PRF): a gold Standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009; 27(1):63-9.
  • 6.Yu P, Zhai Z, Jin X,Yang X, Qi Z. Clinical Application of Platelet-Rich Fibrin in PlasticandReconstructiveSurgery: A SystematicReview. AesthPlastSurg 2018;42:511–519.
  • 7.Kaya F, Kismet K, Ozer H, et al. Can platelet-rich plasma be used safely in intra-abdominal operations? Bratislavske Lekarske Listy. 2016;117(9):525-529. DOI: 10.4149/bll_2016_102.
  • 8.Tutar B, Ekincioglu E, Karaketir S, Berkiten G, Saltürk Z, Arkan ME, Göker AE, Uyar Y. The impact of platelet-rich fibrin (PRF) on olfactory function and pain after septoplasty operations. Eur Arch Otorhinolaryngol. 2020 Apr;277(4):1115-1120. doi: 10.1007/s00405-020-05839-6. Epub 2020 Feb 11. PMID: 32048028.
  • 9.Ural DA, Sarıhan H, Saygın İ, Aykan DA, Ural A, İmamoglu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-222.
  • 10.Aslan GI, Otgun I, Acer T, Tepeoglu M, Hicsonmez A. The effect of intraperitoneal n-acetylcysteine on postoperative adhesions in rat models. Ann Ital Chir. 2017;88:258-262. PMID: 28874629.)
  • 11.Kurt N, Sıkar HE, Kaptanoğlu L, Küçük HF. Separate and synergistic effects of taurolidine and icodextrin in intra-abdominal adhesion prevention. Ulus Travma Acil Cerrahi Derg. 2017 Sep;23(5):377-382.
  • 12.Charboneau AJ, Delaney JP, Beilman G. Fucoidans inhibit the formation of post-operative abdominal adhesions in a rat model. PLoS One. 2018 Nov 21;13(11):e0207797. doi: 10.1371/journal.pone.0207797. Erratum in: PLoS One. 2019 Jan 30;14(1):e0211371. PMID: 30462732; PMCID: PMC6249019.
  • 13.Nagae M, Ikeda T, Mikami Y, Hase H, Ozawa H, Matsuda K, et al. Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Eng. 2007;13(1):147-58.
  • 14.Hinoki A, Saito A, Kinoshita M, Yamamoto J, Saitoh D, Takeoka S. Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model. Br J Surg. 2016 May;103(6):692-700. doi: 10.1002/bjs.10122. Epub 2016 Mar 3. PMID: 26936491.
  • 15.Montalvo-Javé EE, Mendoza-Barrera GE, García-Pineda MA, Jaime Limón ÁR, Montalvo-Arenas C, Castell Rodríguez AE, Tapia Jurado J. Histological Analysis of Intra-Abdominal Adhesions Treated with Sodium Hyaluronate and Carboxymethylcellulose Gel. J Invest Surg. 2016;29(2):80-7. doi: 10.3109/08941939.2015.1076911. Epub 2015 Sep 16. PMID: 26375456.
  • 16.Hu Z, Su Y, Xie P, Chen L, Ji J, Feng T, Wu S, Liang K, Liu Q. OCT angiography-based monitoring of neovascular regression on fibrovascular membrane after preoperative intravitreal conbercept injection. Graefes Arch Clin Exp Ophthalmol. 2019 Aug;257(8):1611-1619. doi: 10.1007/s00417-019-04315-0. Epub 2019 May 3. PMID: 31053944.
  • 17.Altuntaş Z, Gündeşlioğlu AÖ, İnce B, Dadacı M, Savacı N. PlateletRıchPlasma (Prp), PlateletPoorPlasma (Ppp), PlatelerRıchFıbrıne (Prf) Concepts, TheırBıologıcalRoles On WoungHealıngAndClınıcalApplıcatıonsInPlastıcSurgery. TurkPlastSurg 2014;22(2): 49-53. 18. Garg AK. Autologous blood concentrates. Batavia (IL): Quintessence Publishing Co. Inc.; 2018.
  • 19.Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K. Cell-assistedlipotransferforcosmeticbreastaugmentation: supportiveuse of adipose-derivedstem/stromalcells. AesthetPlastSurg2008;32(1):48–55 (discussion 56-47).
  • 20.Miron VE. Microglia-driven regulation of oligodendrocyte lineage cells, myelination, and remyelination. J Leukoc Biol. 2017 May;101(5):1103-1108. doi: 10.1189/jlb.3RI1116-494R. Epub 2017 Mar 1. PMID: 28250011.
  • 21.Kim I, Lee SK, Yoon JI, et al. Fibrin glue improves the therapeutic effect of MSCs by sustaining survival and paracrine function. Tissue Eng Part A 2013; 19(21–22):2373–81.
  • 22.Ponte AL, Marais E, Gallay N, et al. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells 2007;25(7):1737–45.
  • 23.Di Liddo R, Bertalot T, Borean A, et al. Leucocyte and platelet-rich fibrin: a carrier of autologous multipotent cells for regenerative medicine. J Cell Mol Med 2018;22(3):1840–54.
  • 24.Lundquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin. Wound Repair Regen 2008; 16(3):356–63.
  • 25.Ding Y, Cui L, Zhao Q, Zhang W, Sun H, Zheng L. Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice. Ann Plast Surg 2017;79: e15–e19.
  • 26.Turabelidze A., Dipietro L.A. 2012. Inflammation and wound healing. Endod. Top. 24, 26–38.
  • 27.Metzger CE, Narayanan A, Zawieja DC, Bloomfield SA. Inflammatory bowel disease in a rodent model alters osteocyteprotein levels controlling bone turnover. J Bone Miner Res.2017;32(4):802–13
  • 28.Ergüler G., Demir N., Demir R., Structural Propertıes and Functıons of Adhesıon Molecules, Turkiye Klinikleri J Med Sci., 2002;22(3):313-27.)
  • 29.De la Puerta, R., Martinez Dominguez, E., Ruiz-Gutierrez,V. (2000). Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z. Naturforsch., 55,814-819.
  • 30.Cicerale S1,Lucas L,Keast R.Int J Mol Sci. 2010 Feb 2;11(2):458-79.doi: 10.3390/ijms11020458.

Postoperatif peritoneal adhezyonların önlenmesinde saf zeytinyağı ve PRF’nin etkinliği

Year 2022, Volume: 47 Issue: 2, 570 - 579, 30.06.2022
https://doi.org/10.17826/cumj.1031388

Abstract

Amaç: Bu çalışmada, adhezyon oluşması beklenen alanlara uygulanan trombositten zengin fibrin (PRF) ve saf zeytinyağının etkilerinin incelenmesi amaçlanmıştır. PRF’nin adezyona neden olup olmadığının ve PRF'nin batın içi operasyonlarda güvenle kullanıp kullanamayacağını araştırılmaktadır.
Gereç ve Yöntem: Toplamda 4 grup (n=8) olmak üzere 40 adet Wistar albino rat çalışma gruplarında kullanıldı. Çalışma gruplarına dahil edilmeyen 8 rattan PRF eldesi için yararlanıldı. Grup 1’e herhangi bir cerrahi işlem yapılmadı. Adezyon modelin uygulandığı Grup 2’de ilaç kullanılmadı. Adezyon modeli sonrası Grup 3’de 1 cc zeytinyağı ve Grup 4’de 1 cc PRF uygulandı. 21 gün sonra çekum alanları histopatolojik olarak incelendi. Serumda proinflamatuar sitokinlerden tümör nekroz faktör-α (TNF-α), interlökin-6(IL-6), hücreler arası adezyon molekülü-1(ICAM-1) ve platelet-türevli büyüme faktörü(PDGF) düzeyleri ELISA yöntemiyle ölçüldü.
Bulgular: Grup 3 ve 4'te adezyon skorları ve fibrozis şiddeti Grup 2'ye göre anlamlı olarak düşüktü. Plazma TNF-α değeri Grup 2'de Grup 4'e göre anlamlı olarak yüksekti. Plazma PDGF değeri Grup 2'de Grup 3 ve 4'e göre anlamlı olarak yüksekti. .
Sonuç: PRF, fibroblastların ve inflamatuar hücrelerin çoğalmasını engelleyerek ve mezotelyal hücrelerin çoğalmasını teşvik ederek bağırsak yapışmasını azaltabilir. PRF, içeriğindeki büyüme faktörleri ve sitokinlere bağlı olarak antiinflamatuar etkiye sahiptir ve postop adezyonları önler.

References

  • References : 1.Ten Broek RP, Strik C, Issa Y, Bleichrodt RP, van Goor H. Adhesiolysis-related morbidity in abdominal surgery. Ann Surg. 2013 Jul;258(1):98-106. doi: 10.1097/SLA.0b013e31826f4969. PMID: 23013804.
  • 2.Cai T, Qiu J, Ji Y, Li W, Ding Z, Suo C, Chang J, Wang J, He R, Qian Y, Guo X, Zhou L, Sheng H, Shen L, Qiu J. IL-17-producing ST2+ group 2 innate lymphoid cells play a pathogenic role in lung inflammation. J Allergy Clin Immunol. 2019 Jan;143(1):229-244.e9. doi: 10.1016/j.jaci.2018.03.007. Epub 2018 Apr 3. PMID: 29625134; PMCID: PMC6170730.
  • 3.Kırdak T, Uysal E, Korun N. Karın içi yapışıklıkların önlenmesinde metilprednizolonun farklı dozlarının etkinliğinin incelenmesi. Ulus Travma Acil Cerrahi Derg. 2008;14(3):188-91.
  • 4. Khan J, Chandler WL. Discordant Partial Thromboplastin Time (PTT) vs Anti-Xa Heparin Activity. Am J Clin Pathol. 2019 Mar 1;151(4):424-432. doi: 10.1093/ajcp/aqy156. PMID: 30475952.
  • 5.Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. 25. Slowrelease of growthfactorsand thrombospondin-1 in Chouk¬roun’splatelet-rich fibrin (PRF): a gold Standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009; 27(1):63-9.
  • 6.Yu P, Zhai Z, Jin X,Yang X, Qi Z. Clinical Application of Platelet-Rich Fibrin in PlasticandReconstructiveSurgery: A SystematicReview. AesthPlastSurg 2018;42:511–519.
  • 7.Kaya F, Kismet K, Ozer H, et al. Can platelet-rich plasma be used safely in intra-abdominal operations? Bratislavske Lekarske Listy. 2016;117(9):525-529. DOI: 10.4149/bll_2016_102.
  • 8.Tutar B, Ekincioglu E, Karaketir S, Berkiten G, Saltürk Z, Arkan ME, Göker AE, Uyar Y. The impact of platelet-rich fibrin (PRF) on olfactory function and pain after septoplasty operations. Eur Arch Otorhinolaryngol. 2020 Apr;277(4):1115-1120. doi: 10.1007/s00405-020-05839-6. Epub 2020 Feb 11. PMID: 32048028.
  • 9.Ural DA, Sarıhan H, Saygın İ, Aykan DA, Ural A, İmamoglu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-222.
  • 10.Aslan GI, Otgun I, Acer T, Tepeoglu M, Hicsonmez A. The effect of intraperitoneal n-acetylcysteine on postoperative adhesions in rat models. Ann Ital Chir. 2017;88:258-262. PMID: 28874629.)
  • 11.Kurt N, Sıkar HE, Kaptanoğlu L, Küçük HF. Separate and synergistic effects of taurolidine and icodextrin in intra-abdominal adhesion prevention. Ulus Travma Acil Cerrahi Derg. 2017 Sep;23(5):377-382.
  • 12.Charboneau AJ, Delaney JP, Beilman G. Fucoidans inhibit the formation of post-operative abdominal adhesions in a rat model. PLoS One. 2018 Nov 21;13(11):e0207797. doi: 10.1371/journal.pone.0207797. Erratum in: PLoS One. 2019 Jan 30;14(1):e0211371. PMID: 30462732; PMCID: PMC6249019.
  • 13.Nagae M, Ikeda T, Mikami Y, Hase H, Ozawa H, Matsuda K, et al. Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Eng. 2007;13(1):147-58.
  • 14.Hinoki A, Saito A, Kinoshita M, Yamamoto J, Saitoh D, Takeoka S. Polylactic acid nanosheets in prevention of postoperative intestinal adhesion and their effects on bacterial propagation in an experimental model. Br J Surg. 2016 May;103(6):692-700. doi: 10.1002/bjs.10122. Epub 2016 Mar 3. PMID: 26936491.
  • 15.Montalvo-Javé EE, Mendoza-Barrera GE, García-Pineda MA, Jaime Limón ÁR, Montalvo-Arenas C, Castell Rodríguez AE, Tapia Jurado J. Histological Analysis of Intra-Abdominal Adhesions Treated with Sodium Hyaluronate and Carboxymethylcellulose Gel. J Invest Surg. 2016;29(2):80-7. doi: 10.3109/08941939.2015.1076911. Epub 2015 Sep 16. PMID: 26375456.
  • 16.Hu Z, Su Y, Xie P, Chen L, Ji J, Feng T, Wu S, Liang K, Liu Q. OCT angiography-based monitoring of neovascular regression on fibrovascular membrane after preoperative intravitreal conbercept injection. Graefes Arch Clin Exp Ophthalmol. 2019 Aug;257(8):1611-1619. doi: 10.1007/s00417-019-04315-0. Epub 2019 May 3. PMID: 31053944.
  • 17.Altuntaş Z, Gündeşlioğlu AÖ, İnce B, Dadacı M, Savacı N. PlateletRıchPlasma (Prp), PlateletPoorPlasma (Ppp), PlatelerRıchFıbrıne (Prf) Concepts, TheırBıologıcalRoles On WoungHealıngAndClınıcalApplıcatıonsInPlastıcSurgery. TurkPlastSurg 2014;22(2): 49-53. 18. Garg AK. Autologous blood concentrates. Batavia (IL): Quintessence Publishing Co. Inc.; 2018.
  • 19.Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K. Cell-assistedlipotransferforcosmeticbreastaugmentation: supportiveuse of adipose-derivedstem/stromalcells. AesthetPlastSurg2008;32(1):48–55 (discussion 56-47).
  • 20.Miron VE. Microglia-driven regulation of oligodendrocyte lineage cells, myelination, and remyelination. J Leukoc Biol. 2017 May;101(5):1103-1108. doi: 10.1189/jlb.3RI1116-494R. Epub 2017 Mar 1. PMID: 28250011.
  • 21.Kim I, Lee SK, Yoon JI, et al. Fibrin glue improves the therapeutic effect of MSCs by sustaining survival and paracrine function. Tissue Eng Part A 2013; 19(21–22):2373–81.
  • 22.Ponte AL, Marais E, Gallay N, et al. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells 2007;25(7):1737–45.
  • 23.Di Liddo R, Bertalot T, Borean A, et al. Leucocyte and platelet-rich fibrin: a carrier of autologous multipotent cells for regenerative medicine. J Cell Mol Med 2018;22(3):1840–54.
  • 24.Lundquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin. Wound Repair Regen 2008; 16(3):356–63.
  • 25.Ding Y, Cui L, Zhao Q, Zhang W, Sun H, Zheng L. Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice. Ann Plast Surg 2017;79: e15–e19.
  • 26.Turabelidze A., Dipietro L.A. 2012. Inflammation and wound healing. Endod. Top. 24, 26–38.
  • 27.Metzger CE, Narayanan A, Zawieja DC, Bloomfield SA. Inflammatory bowel disease in a rodent model alters osteocyteprotein levels controlling bone turnover. J Bone Miner Res.2017;32(4):802–13
  • 28.Ergüler G., Demir N., Demir R., Structural Propertıes and Functıons of Adhesıon Molecules, Turkiye Klinikleri J Med Sci., 2002;22(3):313-27.)
  • 29.De la Puerta, R., Martinez Dominguez, E., Ruiz-Gutierrez,V. (2000). Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z. Naturforsch., 55,814-819.
  • 30.Cicerale S1,Lucas L,Keast R.Int J Mol Sci. 2010 Feb 2;11(2):458-79.doi: 10.3390/ijms11020458.
There are 29 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research
Authors

Dilan Altıntaş Ural 0000-0002-1976-9122

Duygun Altıntaş Aykan 0000-0001-8224-4006

Muhammed Seyithanoğlu 0000-0001-9680-7535

Abdulkadir Yasir Bahar 0000-0002-6963-3389

Ali Erdal Karakaya 0000-0003-0241-7646

Ahmet Gökhan Güler 0000-0003-4740-3512

Publication Date June 30, 2022
Acceptance Date March 8, 2022
Published in Issue Year 2022 Volume: 47 Issue: 2

Cite

MLA Altıntaş Ural, Dilan et al. “Efficacy of Pure Olive Oil and PRF in the Prevention of Postoperative Peritoneal Adhesions”. Cukurova Medical Journal, vol. 47, no. 2, 2022, pp. 570-9, doi:10.17826/cumj.1031388.